HeliPlex qPCR Multiplex Assay (RUO)

Heliplex Virulence Factor qPCR Kit (RUO)

The HeliPlex qPCR Multiplex Assay is a cutting-edge (Research Use Only) diagnostic tool designed for the rapid and simultaneous detection of 16S and key virulence factors in Helicobacter pylori (H. pylori), a bacterium implicated in various gastric disorders. This real-time quantitative PCR (qPCR) multiplex panel targets three critical genetic markers: vacA s1 allele, vacA m1 allele, and the cagA gene. Utilizing fluorescent probes and primers specific to these regions, the assay enables high-sensitivity identification from gastric biopsy samples, stool, or cultured isolates in a single reaction tube. It delivers results in under 2 hours, with a built-in exogenous internal control for amplification efficiency and contamination prevention. This multiplex format minimizes sample volume requirements and reduces hands-on time, making it ideal for laboratories seeking efficient risk assessment in H. pylori-positive samples. For Research Use Only. Not for use in diagnostic procedures.

Panel Composition

The HeliPlex panel detects Helicobacter pylori and three key virulence markers — cagA, vacA s1, and vacA m1 — in a single well, with Bacillus atrophaeus as an exogenous internal control.

Multiplex Kits

  • H. pylori 16S rRNA
  • cagA
  • vacA m1
  • vacA s1
  • Internal Control (B. atrophaeus)

Technical Specifications

Total targets
Reactions
Modules
Module composition
Mastermix
Optical channels
Internal Control
Internal Control (B. atrophaeus)
Shipping
Next Day Shipping, Dry Ice
Storage
Store at -20°C, minimize freeze/thaw cycles
Instrument Compatibility
QuantStudio 5, 6, 7, 12; Bio-Rad CFX Opus 96 & CFX96 Touch
*Technical Specifications related to product performance have been obtained by A2K or other clinical laboratories and are not guaranteed. Performance specifications are unique to each laboratory, it's instruments, and it's staff.