Heliplex Virulence Factor qPCR Kit (RUO)
The HeliPlex qPCR Multiplex Assay is a cutting-edge (Research Use Only) diagnostic tool designed for the rapid and simultaneous detection of 16S and key virulence factors in Helicobacter pylori (H. pylori), a bacterium implicated in various gastric disorders. This real-time quantitative PCR (qPCR) multiplex panel targets three critical genetic markers: vacA s1 allele, vacA m1 allele, and the cagA gene. Utilizing fluorescent probes and primers specific to these regions, the assay enables high-sensitivity identification from gastric biopsy samples, stool, or cultured isolates in a single reaction tube. It delivers results in under 2 hours, with a built-in exogenous internal control for amplification efficiency and contamination prevention. This multiplex format minimizes sample volume requirements and reduces hands-on time, making it ideal for laboratories seeking efficient risk assessment in H. pylori-positive samples.
Valuable Insights
Detecting vacA s1, vacA m1, and cagA in H. pylori infections provides critical prognostic information, as these factors correlate with the progression from asymptomatic colonization to severe gastroduodenal diseases such as chronic gastritis, peptic ulcers (gastric or duodenal), and gastric adenocarcinoma. H. pylori infects over half the global population, but only a subset develops complications; virulence genotyping helps identify high-risk patients who may benefit from aggressive eradication therapy, endoscopic surveillance, or chemoprevention strategies.
Correlation with Disease
Numerous studies, including meta-analyses and population-based research, demonstrate strong associations between these virulence factors and disease outcomes. Strains harboring cagA, vacA s1, and vacA m1 (especially in combination) are linked to higher odds of severe pathology, while their absence correlates with milder or asymptomatic infections.
Kit Contents
Included:
- HeliPlex Assay
- PureAmp Express Mastermix
- User Guide
- Instrument Template
Not Included:
- A2K HeliPlex Amplification Control
- A2K Spectral Calibration Plates
Technical Specifications
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Module 1
16S
VacA s1
VacA m1
CagA
Exogenous Control (B. atrophaeus)
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A2K Evaluation
✓ Analytical Controls
✓ Quantitative Genomic Material
✓ Clinical Samples
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Optical Channels
- FAM
- SUN
- TAMRA
- TEXAS RED
- ATTO647
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Shipping
- 1-3 Business Days
- Conditions: Dry Ice
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Storage
- Store at -20°C
- Minimize freeze thaws