
Heliplex Virulence Factor qPCR Kit (RUO)
The HeliPlex qPCR Multiplex Assay is a cutting-edge (Research Use Only) diagnostic tool designed for the rapid and simultaneous detection of 16S and key virulence factors in Helicobacter pylori (H. pylori), a bacterium implicated in various gastric disorders. This real-time quantitative PCR (qPCR) multiplex panel targets three critical genetic markers: vacA s1 allele, vacA m1 allele, and the cagA gene. Utilizing fluorescent probes and primers specific to these regions, the assay enables high-sensitivity identification from gastric biopsy samples, stool, or cultured isolates in a single reaction tube. It delivers results in under 2 hours, with a built-in exogenous internal control for amplification efficiency and contamination prevention. This multiplex format minimizes sample volume requirements and reduces hands-on time, making it ideal for laboratories seeking efficient risk assessment in H. pylori-positive samples. For Research Use Only. Not for use in diagnostic procedures.
Panel Composition
The HeliPlex panel detects Helicobacter pylori and three key virulence markers — cagA, vacA s1, and vacA m1 — in a single well, with Bacillus atrophaeus as an exogenous internal control.
Multiplex Kits
- H. pylori 16S rRNA
- cagA
- vacA m1
- vacA s1
- Internal Control (B. atrophaeus)
Technical Specifications
- Total targets
- Reactions
- Modules
- Module composition
- Mastermix
- Optical channels
- Internal Control
- Internal Control (B. atrophaeus)
- Shipping
- Next Day Shipping, Dry Ice
- Storage
- Store at -20°C, minimize freeze/thaw cycles
- Instrument Compatibility
- QuantStudio 5, 6, 7, 12; Bio-Rad CFX Opus 96 & CFX96 Touch