Heliplex Virulence Factor qPCR Kit (RUO)
The HeliPlex qPCR Multiplex Assay is a cutting-edge (Research Use Only) diagnostic tool designed for the rapid and simultaneous detection of 16S and key virulence factors in Helicobacter pylori (H. pylori), a bacterium implicated in various gastric disorders. This real-time quantitative PCR (qPCR) multiplex panel targets three critical genetic markers: vacA s1 allele, vacA m1 allele, and the cagA gene. Utilizing fluorescent probes and primers specific to these regions, the assay enables high-sensitivity identification from gastric biopsy samples, stool, or cultured isolates in a single reaction tube. It delivers results in under 2 hours, with a built-in exogenous internal control for amplification efficiency and contamination prevention. This multiplex format minimizes sample volume requirements and reduces hands-on time, making it ideal for laboratories seeking efficient risk assessment in H. pylori-positive samples.
Modules
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Module 1
16S
VacA s1
VacA m1
cagA