PureAmp Pro Mastermix (RT-qPCR)
The A2K PureAmp Pro RT-qPCR Master Mix is a convenient and pre-formulated 2X concentrated master mix created specifically for probe-based real-time quantitative PCR. Featuring an antibody-mediated hot-start DNA polymerase, reverse transcriptase, dNTPs, MgCl2, enhancers, and stabilizers, PureAmp Pro combines reverse transcription and amplification in a single closed-tube reaction. It offers a streamlined, one-step solution for efficient and reliable results directly from RNA or DNA templates. PureAmp Pro is the master mix used across A2K's respiratory pathogen panels, including the Respiratory Pathogen Panel and Respiratory Pathogen Panel Select, delivering consistent performance in demanding multiplex workflows.
Why PureAmp Pro
Select a criterion to see how PureAmp Pro is built to perform in one-step RT-qPCR - from formulation to the panels it powers.
A ready-to-use, pre-formulated master mix
PureAmp Pro ships as a 2X concentrate containing an antibody-mediated hot-start DNA polymerase, reverse transcriptase, dNTPs, MgCl2, enhancers, and stabilizers - everything needed for one-step RT-qPCR except your assay and template.
Reverse transcription and amplification in a single reaction
PureAmp Pro's built-in reverse transcriptase means RNA templates are reverse-transcribed and amplified in the same closed-tube reaction - no separate cDNA synthesis step, reducing hands-on time and contamination risk.
Built for singleplex and multiplex assays
PureAmp Pro amplifies singleplex and multiplex reactions up to 5-plex, making it well suited to high-throughput screening where multiple targets are detected in a single well.
Compatible with fast and standard cycling
A fixed 15-minute reverse transcription step at 50°C is followed by polymerase activation and either fast or standard PCR cycling - no reformulation or reoptimization required when switching instruments or protocols.
The master mix behind A2K's pathogen panels
PureAmp Pro is the master mix included in A2K's Respiratory Pathogen Panel, Respiratory Pathogen Panel Select, Influenza A H5 (Clade 2.3.4.4b) PureAmp Kit, and Gastrointestinal Pathogen Panel, so performance in your standalone RT-qPCR assays matches what's validated across those panels.
Compatible with A2K's full optical channel set
PureAmp Pro performs across the same five optical channels used throughout A2K's multiplex panel line, giving assay designers a consistent spectral palette to build against.
Simple, consistent handling
PureAmp Pro ships on dry ice and should be stored at -15°C to -30°C in a constant-temperature, non-frost-free freezer, minimizing freeze-thaw cycles to preserve enzyme activity and reaction consistency lot to lot.
Scales from bench to high-throughput
PureAmp Pro is available in 1 mL, 5 mL, and 25 mL formats, so labs can match order volume to reaction throughput without overbuying.
- SKU
- MM-PA-1