PureAmp Express Mastermix (qPCR)
The A2K PureAmp Express qPCR Master Mix is a convenient and pre-formulated 2X concentrated master mix created specifically for probe-based real-time quantitative PCR. Featuring an antibody-mediated hot-start DNA polymerase, dNTPs, MgCl2, enhancers, and stabilizers, PureAmp Express is designed for applications involving purified nucleic acid in qPCR. It offers a streamlined, one-step solution for efficient and reliable results in real-time quantitative PCR assays. PureAmp Express is the master mix used across A2K's multiplex pathogen detection panels, including the UTI Panel, delivering consistent performance in demanding multiplex workflows.
Why PureAmp Express
Select a criterion to see how PureAmp Express is built to perform in real-time qPCR - from formulation to the panels it powers.
A ready-to-use, pre-formulated master mix
PureAmp Express ships as a 2X concentrate containing an antibody-mediated hot-start DNA polymerase, dNTPs, MgCl2, enhancers, and stabilizers - everything needed for probe-based real-time qPCR except your assay and template.
Built for singleplex and multiplex assays
PureAmp Express amplifies singleplex and multiplex reactions up to 5-plex, making it well suited to high-throughput screening where multiple targets are detected in a single well.
Compatible with fast and standard cycling
Whether your lab runs fast or standard cycling protocols, PureAmp Express performs consistently across both - no reformulation or reoptimization required when switching instruments or protocols.
The master mix behind A2K's multiplex panels
PureAmp Express is the master mix included in A2K's UTI Panel, Nail Pathogens Panel, Wound Pathogens Panel, Antibiotic Resistance Panel, Terbinafine Resistance Panel, and HeliPlex Virulence Kit, so performance in your standalone qPCR assays matches what's validated across those panels.
Compatible with A2K's full optical channel set
PureAmp Express performs across the same five optical channels used throughout A2K's multiplex panel line, giving assay designers a consistent spectral palette to build against.
Simple, consistent handling
PureAmp Express ships cold and should be stored at -20°C with minimal freeze-thaw cycling to preserve enzyme activity and reaction consistency lot to lot.
Scales from bench to high-throughput
PureAmp Express is available in 1 mL, 5 mL, and 25 mL formats, so labs can match order volume to reaction throughput without overbuying.
- SKU
- MM-PAEX-1