The A2K Direct Amp Master Mix is a pre-prepared, one-step, 4X concentrated master mix tailored for probe-based real-time quantitative PCR (qPCR). It incorporates a mutant hot-start reverse transcriptase, a mutant hot-start DNA polymerase, dNTPs, MgCl2, enhancers, and stabilizers. Additionally, an optional enhancer solution is included for neutralizing PCR inhibitors, such as nucleases in crude samples. This versatile product is suitable for direct amplification of both crude samples and purified nucleic acid in PCR applications.